Journal: iScience

Article Title: Identification of optimal conditions for human placental explant culture and extracellular vesicle release

doi: 10.1016/j.isci.2023.108046

Figure Lengend Snippet:

Article Snippet: Human anti-CD41 PE , Cellarcus Biosciences , Cat# CBS71-PE-100T.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Proliferation Assay, LDH Cytotoxicity Assay, Software

Journal: Blood

Article Title: Expansion of the neonatal platelet mass is achieved via an extension of platelet lifespan

doi: 10.1182/blood-2013-06-508200

Figure Lengend Snippet: Megakaryopoiesis during murine neonatal development. MK concentration (A) and MK diameter (B) were measured in the liver (orange triangles and solid line), BM (green diamonds and interrupted line), and spleen (blue squares and solid line) of newborn mice on days P1, P3, P5, P7, P10, and P14, and in adult mice (>60 days) after immunohistochemical staining with anti-VWF. Data presented are means ± SD from at least 4 mice at each time point. (A) MK concentration, expressed as number of MKs present in an area of tissue measuring 250 × 250 µm at ×400. (B) MK diameter (in μm) measured under the microscope at ×400. (C) MK maturation was analyzed by immunofluorescent staining for VWF (green) and PF4 (red). Shown are representative neonatal MKs (spleen, upper row) and adult MKs (BM, lower row). Note a blood vessel in the upper panel, with visible endothelial cells expressing only VWF (green). All photomicrographs were obtained at ×400, Bar: 10 μm. (D) MKs isolated from the spleen of newborn mice (P5-P10, top) were compared with MKs from the spleen of adult mice (bottom). The ploidy of CD41/CD61 double-positive cells was determined by flow cytometry after staining with Hoechst 33342. (E) Ultrastructure was analyzed by electron microscopy in flow-sorted 2-4N MKs isolated from the spleen of neonatal mice (P5-P10). Despite their small size, ∼30% of 2-4N neonatal MKs were mature, as indicated by the presence of an open demarcation membrane system. (Bar: 2 μm)

Article Snippet: 23 Diluted samples were labeled with PE-conjugated anti-mouse CD41, and were mixed with a known number of FITC-labeled SPHERO beads (Spherotech, Libertyville, IL).

Techniques: Concentration Assay, Immunohistochemical staining, Staining, Microscopy, Expressing, Isolation, Flow Cytometry, Electron Microscopy

Journal: Blood

Article Title: Expansion of the neonatal platelet mass is achieved via an extension of platelet lifespan

doi: 10.1182/blood-2013-06-508200

Figure Lengend Snippet: Prolonged in vitro survival of human neonatal platelets. In vitro survival of human neonatal and adult platelets was evaluated by incubating PRP from human umbilical CB and adult PB at 37°C for 5 days. (A) Annexin V-binding and P-selectin expression were evaluated daily by flow cytometry in CD41-gated CB and PB platelets during a 5-day incubation period. (B) Percentages of Annexin V positive platelets were quantified daily and compared between CB-PRP (n = 6) and PB-PRP (n = 8). (C) Platelet survival was quantified daily by FACS analysis after staining with the mitochondrial dye JC-1. The percentages of live platelets were measured in CB-PRP (n = 3) compared with PB-PRP (n = 5). *P < .05; **P < .01; ***P < .001.

Article Snippet: 23 Diluted samples were labeled with PE-conjugated anti-mouse CD41, and were mixed with a known number of FITC-labeled SPHERO beads (Spherotech, Libertyville, IL).

Techniques: In Vitro, Binding Assay, Expressing, Flow Cytometry, Incubation, Staining